The 'Single Ladies' singer ' who will perform on the show's Pyramid Stage as the closing act of the UK festival in Somerset, South West England, on Sunday 26 June - has been feeling anxious about the show, so has secretly rented a studio in London to put in extra rehearsals.
A source told the Daily Mirror newspaper: "Beyonce is so nervous about the gig she has booked the space for two weeks so she can perfect the set, which will include dizzying pyrotechnics. She's literally playing with fire.
'Her performance at Glastonbury is already looking epic. She's been given a helping hand by Jay-Z and Kanye West who both look set to perform songs with her.'
The 29-year-old singer will also spend the fortnight with her back-up singers, dancers, sound engineers and lighting technicians as she pulls out all the stops for her performance.
Beyonce admitted earlier this year she "cannot wait" to take to the stage at the spectacle.
She said: "This really is the biggest festival in the world and I cannot wait to perform there. Everyone who attends is really appreciative of music and is in such a good mood that entire weekend.
"I'm pumped just thinking about that huge audience and soaking up their energy."
Beyonce was last at Glastonbury in 2008 with Jay, her husband, who was headlining that year.